{"id":527221,"date":"2025-11-21T09:21:38","date_gmt":"2025-11-21T09:21:38","guid":{"rendered":"https:\/\/veteo.eu\/?page_id=527221"},"modified":"2026-04-29T11:46:36","modified_gmt":"2026-04-29T11:46:36","slug":"cytometry","status":"publish","type":"page","link":"https:\/\/veteo.eu\/en\/services\/cytometry\/","title":{"rendered":"Flow cytometry"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row full_width=&#8221;stretch_row&#8221; gap=&#8221;35&#8243; content_placement=&#8221;middle&#8221; el_id=&#8221;start&#8221; el_class=&#8221;en-st-pt&#8221;][vc_column width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1764926528107{padding-top: 0px !important;}&#8221;][vc_column_text css=&#8221;&#8221;]<\/p>\n<h2 class=\"mainowy\">Immunophenotyping &#8211; a flow cytometry based method now available to Veteo clients<\/h2>\n<p>The Veteo Laboratory, in cooperation with a team of veterinary oncologists and scientists, offers immunophenotyping of cells using flow cytometry.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1764926534225{padding-top: 0px !important;}&#8221;][vc_single_image image=&#8221;527225&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; equal_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; css=&#8221;.vc_custom_1615022529078{padding-top: 60px !important;padding-bottom: 60px !important;background-color: #fbfbfb !important;}&#8221;][vc_column width=&#8221;4\/12&#8243;][vc_single_image image=&#8221;527228&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][\/vc_column][vc_column width=&#8221;8\/12&#8243;][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3>The founder and supervisor of the project is Dr. Dariusz Jagielski.<\/h3>\n<p><em>\u201cAt the beginning of my work with oncology patients, almost twenty years ago, I became interested in immunophenotyping and flow cytometry and was fascinated by the potential of this method. In the end, we managed to gather a group of people who offer cytometry as a standard, commercially available test for patients with cancers of the hematopoietic system.<\/em><br data-start=\"430\" data-end=\"433\" \/><em>The key to success is contact with the attending veterinarian \u2013 this leads to obtaining additional useful clinical insights from the cytometric examination.\u201d<\/em>[\/vc_column_text][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; equal_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; css=&#8221;.vc_custom_1615022555609{padding-top: 60px !important;padding-bottom: 60px !important;}&#8221;][vc_column width=&#8221;4\/12&#8243;][vc_single_image image=&#8221;527231&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][\/vc_column][vc_column width=&#8221;8\/12&#8243;][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3>Our team<\/h3>\n<p>Our team consists of qualified staff under the supervision of an experienced leader, <strong>dr n. med. lek. wet. <\/strong><strong>Marta Idziak<\/strong>, a specialist in veterinary laboratory diagnostics.[\/vc_column_text][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; equal_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; css=&#8221;.vc_custom_1768465434283{margin-bottom: 35px !important;}&#8221;][vc_column width=&#8221;4\/12&#8243;][vc_single_image image=&#8221;527234&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][\/vc_column][vc_column width=&#8221;8\/12&#8243;][vc_column_text css=&#8221;&#8221;]Scientific support is provided by <strong>dr. Aleksandra Pawlak<\/strong> from the Faculty of Veterinary Medicine at the University of Environmental and Life Sciences in Wroc\u0142aw.<\/p>\n<p><em>\u201cFlow cytometry is one of the fundamental scientific methods I use in my work. Cytometric immunophenotyping of canine lymphomas and leukemias was one part of my doctoral thesis, and since then I have been a true enthusiast of this diagnostic method, continually developing my expertise in its use.<\/em><br data-start=\"463\" data-end=\"466\" \/><em>In my opinion, the commercial availability of this examination is the pinnacle of my scientific work \u2014 because science should serve practice!\u201d<\/em>[\/vc_column_text][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; bg_type=&#8221;image&#8221; parallax_style=&#8221;vcpb-vz-jquery&#8221; bg_image_new=&#8221;id^1766|url^https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/03\/immunofenotypizacja.jpg|caption^null|alt^null|title^immunofenotypizacja|description^null&#8221; bg_image_repeat=&#8221;no-repeat&#8221; css=&#8221;.vc_custom_1615025856523{padding-top: 60px !important;padding-bottom: 60px !important;background-image: url(https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/03\/immunofenotypizacja.jpg?id=1766) !important;}&#8221;][vc_column][vc_column_text css=&#8221;&#8221; el_class=&#8221;whiter&#8221;]<\/p>\n<h3><span style=\"color: #ffffff;\">We invite veterinarians with an interest in veterinary oncology who wish to advance their hematologic and oncologic diagnostic capabilities to collaborate with us.<\/span><\/h3>\n<p><span style=\"color: #ffffff;\">We are also open to cooperation with research centers.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1615026061175{padding-top: 60px !important;padding-bottom: 90px !important;}&#8221; el_id=&#8221;cytometria-przeplywowa&#8221;][vc_column width=&#8221;2\/3&#8243;][vc_column_text css=&#8221;.vc_custom_1768466765531{margin-bottom: 0px !important;}&#8221;]<\/p>\n<h2>Flow cytometry<\/h2>\n<p><span data-teams=\"true\">Flow cytometry is a method widely used in laboratory diagnostics. By detecting laser light scanning a cell suspension, it enables precise assessment of both the number of cells and their morphological characteristics. Since the beginning of our activity, we have been using the most advanced veterinary flow cytometers available on the market, offering professional analyses of hematological parameters in many animal species.<\/span><\/p>\n<p><span data-teams=\"true\">Currently, as the only laboratory in Poland, we combine flow cytometry technology with monoclonal antibodies and fluorescence, allowing us to perform advanced immunophenotyping of cells. This approach enables the detection of differentiation antigens (clusters of differentiation, CD) on the surface and inside cells, providing precise determination of their origin and characteristics.<\/span>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;528079&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h2>Indications for immunophenotyping<\/h2>\n<p>Immunophenotyping in veterinary medicine is used primarily in small animal hemato-oncology.<br \/>\nIn the early years of our activity, this test was offered exclusively to canine patients. It is now also available for cats.<br \/>\nIn addition, we have expanded our offer to include Ki-67 antigen testing in dogs \u2013 a marker of cell proliferation that is helpful in assessing tumor aggressiveness.[\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1768386269394{margin-bottom: 0px !important;}&#8221;]<\/p>\n<h3>The main indications for immunophenotyping by flow cytometry in dogs and cats include:<\/h3>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1615026061175{padding-top: 60px !important;padding-bottom: 90px !important;}&#8221;][vc_column][vc_row_inner equal_height=&#8221;yes&#8221; gap=&#8221;20&#8243;][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1768385891871{padding-top: 50px !important;padding-right: 50px !important;padding-bottom: 50px !important;padding-left: 50px !important;background-color: #F2F1F5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768386303988{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>differentiation between reactive and neoplastic lymphoproliferative processes (lymphocytosis\/reactive lymph node vs. leukemia\/lymphoma)<\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1768385914425{padding-top: 50px !important;padding-right: 50px !important;padding-bottom: 50px !important;padding-left: 50px !important;background-color: #D0CED7 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768386399358{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>differentiation between acute and chronic leukemias (note: dogs only)<\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner equal_height=&#8221;yes&#8221; gap=&#8221;20&#8243;][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1768385891871{padding-top: 50px !important;padding-right: 50px !important;padding-bottom: 50px !important;padding-left: 50px !important;background-color: #F2F1F5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768386329718{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>differentiation of lymphoma cell origin (T-cell vs. B-cell)<\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1768385914425{padding-top: 50px !important;padding-right: 50px !important;padding-bottom: 50px !important;padding-left: 50px !important;background-color: #D0CED7 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768386421141{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>differentiation of leukemia cell origin (lymphoid vs. myelomonocytic)<\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner equal_height=&#8221;yes&#8221; gap=&#8221;20&#8243;][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1768385891871{padding-top: 50px !important;padding-right: 50px !important;padding-bottom: 50px !important;padding-left: 50px !important;background-color: #F2F1F5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768386350894{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>assessment of prognostic parameters in lymphoma\/leukemia (phenotype, aberrations, MHC II molecule expression)<\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1768385914425{padding-top: 50px !important;padding-right: 50px !important;padding-bottom: 50px !important;padding-left: 50px !important;background-color: #D0CED7 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768386429735{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>differentiation of low- and high-grade lymphomas (Ki-67; note: dogs only)<\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner equal_height=&#8221;yes&#8221; gap=&#8221;20&#8243;][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1768385891871{padding-top: 50px !important;padding-right: 50px !important;padding-bottom: 50px !important;padding-left: 50px !important;background-color: #F2F1F5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768386366712{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>definitive confirmation of T-zone lymphoma (note: dogs only)<\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1768385914425{padding-top: 50px !important;padding-right: 50px !important;padding-bottom: 50px !important;padding-left: 50px !important;background-color: #D0CED7 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768386448269{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>differentiation between thymoma and mediastinal lymphoma (note: dogs only)<\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; css=&#8221;.vc_custom_1615024486808{padding-top: 60px !important;padding-bottom: 60px !important;background-color: #fafafa !important;}&#8221;][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3>The panel of monoclonal antibodies we use includes:<\/h3>\n<ul>\n<li>in dogs, antibodies against all of the following markers<\/li>\n<li>in cats, only antibodies directed against the markers indicated <img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-353604\" src=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cytometry-icon.png\" alt=\"Cytometry for cats\" width=\"22\" height=\"22\" \/><\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_row_inner equal_height=&#8221;yes&#8221; gap=&#8221;20&#8243;][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024531510{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768466440107{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD45 \/ CD18 <img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-353604\" src=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cytometry-icon.png\" alt=\"Cytometry for cats\" width=\"45\" height=\"45\" \/><\/h3>\n<p>leukocyte marker[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024539797{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1763718965077{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD34<\/h3>\n<p>stem cell marker[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024548564{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1763718989497{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD3<\/h3>\n<p>T-cell marker (surface)[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner equal_height=&#8221;yes&#8221; gap=&#8221;20&#8243;][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024531510{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1763719005727{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD3c<\/h3>\n<p>T-cell marker (intracellular)[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024539797{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768400649495{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD4 <img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-353604\" src=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cytometry-icon.png\" alt=\"Cytometry for cats\" width=\"45\" height=\"45\" \/><\/h3>\n<p>T-cell helper marker[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024548564{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768400658112{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD8 <img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-353604\" src=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cytometry-icon.png\" alt=\"Cytometry for cats\" width=\"45\" height=\"45\" \/><\/h3>\n<p>cytotoxic T-cell marker[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner equal_height=&#8221;yes&#8221; gap=&#8221;20&#8243;][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024531510{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768400665581{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD5 <img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-353604\" src=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cytometry-icon.png\" alt=\"Cytometry for cats\" width=\"45\" height=\"45\" \/><\/h3>\n<p>T-cell marker[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024539797{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768400672515{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD21 <img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-353604\" src=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cytometry-icon.png\" alt=\"Cytometry for cats\" width=\"45\" height=\"45\" \/><\/h3>\n<p>mature B-cell marker (surface)[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024548564{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768400684726{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD79\u03b1\u200b<\/h3>\n<p>B-cell marker (intracellular)[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner equal_height=&#8221;yes&#8221; gap=&#8221;20&#8243;][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024531510{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768400725936{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD14<img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-353604\" src=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cytometry-icon.png\" alt=\"Cytometry for cats\" width=\"45\" height=\"45\" \/><\/h3>\n<p>monoidal line marker[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024539797{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1763719139266{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>MHCII<\/h3>\n<p>major histocompatibility complex class II marker (present on lymphocytes and macrophages)[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024548564{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768400842546{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>CD11\u200bb <img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-353604\" src=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cytometry-icon.png\" alt=\"Cytometry for cats\" width=\"45\" height=\"45\" \/><\/h3>\n<p>myelomonocytic line marker[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner equal_height=&#8221;yes&#8221; gap=&#8221;20&#8243;][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024531510{padding: 25px !important;background-color: #f2f1f5 !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1768400884059{margin-top: 0px !important;margin-right: 0px !important;margin-bottom: 0px !important;margin-left: 0px !important;padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;]<\/p>\n<h3>Ki-67<\/h3>\n<p>cell proliferation activity marker[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1615024843798{padding-top: 25px !important;padding-right: 25px !important;padding-bottom: 25px !important;padding-left: 25px !important;}&#8221;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1615026071228{padding-top: 60px !important;padding-bottom: 90px !important;}&#8221; el_id=&#8221;wazne-informacje&#8221;][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h2 class=\"mainowy\">Important notice<\/h2>\n<p>[\/vc_column_text]<div class=\"standard-arrow list-divider bullet-top\"><ul>\n<li>Immunophenotyping of cells using flow cytometry requires <strong>maintaining high cell viability<\/strong>. For this reason, the analysis must be performed no later than<strong> 48\u201372 hours<\/strong> after collection of the cellular material.<\/li>\n<li>In the case of flow cytometric analysis of lymph node biopsies and solid organs, preparation of a cell suspension using a dedicated medium is required (<a href=\"#material-for-examination\">see Instructions<\/a>). This requirement does not apply to blood, bone marrow, or body cavity fluids, which are ready-to-analyze cell suspensions.<\/li>\n<li>Cellular material must not be frozen or fixed.<\/li>\n<li>Samples for flow cytometric testing <strong>are accepted from Monday to Thursday<\/strong> (analyses are not performed on weekends or public holidays).<\/li>\n<li>Medications, particularly cytostatics and glucocorticosteroids, can significantly affect immunophenotyping results. Therefore, <strong>it is recommended to perform the analysis prior to initiating therapy<\/strong>.<\/li>\n<li>Immunophenotyping results cannot be interpreted without knowledge of the cytopathological features of the analyzed material and the patient\u2019s clinical data. Therefore, it is necessary to <strong>complete the <a href=\"https:\/\/veteo.eu\/en\/cytometry-examination-questionnaire\/\">online questionnaire<\/a> and submit fresh samples and\/or cytological test results<\/strong>.<\/li>\n<\/ul>\n<\/div>[\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; css=&#8221;.vc_custom_1763720435859{padding-top: 60px !important;padding-bottom: 60px !important;background-color: #fafafa !important;}&#8221; el_id=&#8221;material-for-examination&#8221;][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h2 class=\"mainowy\">Material for examination<\/h2>\n<p>Any type of <strong>liquid suspension of viable cells<\/strong> can be used for immunophenotyping by flow cytometry.<br \/>\nIf peripheral blood, bone marrow, or body cavity fluid is to be analyzed, the material should be collected into a tube with anticoagulant (as for a complete blood count) and sent to the laboratory.<br \/>\nIn the case of biopsies from lymph nodes and other solid organs, it is necessary to suspend the cells in <strong>a specialized transport medium<\/strong>.<\/p>\n<h3><span style=\"color: #b30000;\">Instructions for preparing the transport medium for lymph node\/solid organ biopsies<\/span><\/h3>\n<ol>\n<li>Collect peripheral blood from the patient undergoing biopsy into an EDTA tube.<\/li>\n<li>Centrifuge the collected blood sample to obtain plasma.<\/li>\n<li>Mix the plasma with sterile 0.9% NaCl solution at a ratio of 1:9. Approximately 2 ml of medium is sufficient for one patient sample (1.8 ml of 0.9% NaCl + 0.2 ml of plasma).<\/li>\n<li>Place the cell sample in the prepared medium.<\/li>\n<\/ol>\n<p><strong>The minimum cellularity<\/strong> required to perform the analysis is approximately 3 million viable cells. The sample volume to be sent for analysis depends on the cell count in the tested tissue and is difficult to specify precisely (typically, 2\u20133 lymph node biopsy cores, about 2\u20133 ml of peripheral blood, or 1\u20132 ml of bone marrow are sufficient).<\/p>\n<p>Along with the cellular material, it is advisable to provide blood smears\/cytological slides and\/or current cytology results.[\/vc_column_text][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; equal_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; bg_type=&#8221;image&#8221; parallax_style=&#8221;vcpb-vz-jquery&#8221; bg_image_new=&#8221;id^1794|url^https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/03\/ankeita-cytomatria.jpg|caption^null|alt^null|title^ankeita-cytomatria|description^null&#8221; parallax_sense=&#8221;42&#8243; css=&#8221;.vc_custom_1763720394804{padding-top: 60px !important;padding-bottom: 60px !important;}&#8221; el_id=&#8221;questionnaire&#8221;][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h2 style=\"text-align: center;\"><span style=\"color: #ffffff;\">Questionnaire for the referring veterinarian<\/span><\/h2>\n<p>[\/vc_column_text][vc_btn title=&#8221;Examination questionnaire&#8221; style=&#8221;flat&#8221; shape=&#8221;square&#8221; color=&#8221;black&#8221; size=&#8221;lg&#8221; align=&#8221;center&#8221; css_animation=&#8221;bounceIn&#8221; css=&#8221;&#8221; link=&#8221;url:https%3A%2F%2Fveteo.eu%2Fen%2Fservices%2Fcytometry%2Fquestionnaire%2F|title:Cytometria%20%E2%80%93%20Ankieta%20do%20badania&#8221;][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; css=&#8221;.vc_custom_1763720469536{padding-top: 60px !important;padding-bottom: 60px !important;}&#8221;][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h2 class=\"mainowy\">Interesting cases<\/h2>\n<h3 class=\"subber\">Below are brief case reports that demonstrate the diagnostic potential of immunophenotypic testing of canine cells using flow cytometry.<\/h3>\n<p>[\/vc_column_text][vc_toggle title=&#8221;Schnauzer with clinical suspicion of lymphoma&#8221; css=&#8221;&#8221;]<\/p>\n<ul>\n<li>A 10-year-old Schnauzer with clinical suspicion of lymphoma confirmed by cytopathological examination of the lymph nodes.<\/li>\n<li>To assess the clonality and origin of the neoplastic cells, a PARR test was performed, which surprisingly yielded a negative result (features of polyclonal proliferation of T and B lymphocytes were found, as seen in non-neoplastic stimulation of lymph nodes).<\/li>\n<li><span style=\"font-weight: 400;\">The results of the cytometric analysis showed that the dominant population in the examined lymph node consisted of medium to large cells with the immunophenotype CD45+ CD21+ CD79\u03b1+ MHC II+. This finding indicated a disruption of the physiological proportions between T and B lymphocytes in the lymph node and clearly suggested excessive proliferation of B lymphocytes.*<\/span><\/li>\n<\/ul>\n<p><strong>Taking into account the clinical data and the cytology results of the patient\u2019s lymph nodes, immunophenotyping made it possible to resolve doubts about the final diagnosis \u2014 the presence of B-cell lymphoma was confirmed and appropriate therapy was initiated.<\/strong><\/p>\n<p><em>*Pawlak A, et al. Immunophenotypic characterization of canine malignant lymphoma: a retrospective study of cases diagnosed in Poland Lower Silesia, over the period 2011\u20132013. Veterinary and Comparative Oncology, 2014, 14, 52\u201359.<\/em><\/p>\n<p>&nbsp;<\/p>\n<p><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/1.1.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2229 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/1.1-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/1.2.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2230 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/1.2-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/1.3.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2228 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/1.3-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/a>[\/vc_toggle][vc_toggle title=&#8221;Shih Tzu with signs of generalized lymphadenopathy and marked lymphocytosis in peripheral blood&#8221; css=&#8221;&#8221;]<\/p>\n<ul>\n<li>An 11-year-old female Shih Tzu with signs of generalized lymphadenopathy and marked lymphocytosis in peripheral blood persisting for about a year.<\/li>\n<li>Histopathological examination of the excised submandibular lymph node performed at the beginning of the diagnostic process indicated reactive hyperplasia, which did not correlate with the clinical picture.<\/li>\n<li><span style=\"font-weight: 400;\">The immunophenotyping result indicated the presence of a clearly dominant population of cells in the examined lymph node with the immunophenotype CD45- CD3+ CD8+ CD5+ MHC II+. The presence of a large population of cells with the phenotype of cytotoxic T lymphocytes lacking expression of the lymphocyte marker (CD45) was indicative of their neoplastic transformation.<\/span><\/li>\n<\/ul>\n<article class=\"text-token-text-primary w-full focus:outline-none [--shadow-height:45px] has-data-writing-block:pointer-events-none has-data-writing-block:-mt-(--shadow-height) has-data-writing-block:pt-(--shadow-height) [&amp;:has([data-writing-block])&gt;*]:pointer-events-auto [content-visibility:auto] supports-[content-visibility:auto]:[contain-intrinsic-size:auto_100lvh] scroll-mt-[calc(var(--header-height)+min(200px,max(70px,20svh)))]\" dir=\"auto\" tabindex=\"-1\" data-turn-id=\"request-WEB:5df8c942-5443-47bc-92e0-ac9837bd0a5d-79\" data-testid=\"conversation-turn-160\" data-scroll-anchor=\"true\" data-turn=\"assistant\">\n<div class=\"text-base my-auto mx-auto pb-10 [--thread-content-margin:--spacing(4)] thread-sm:[--thread-content-margin:--spacing(6)] thread-lg:[--thread-content-margin:--spacing(16)] px-(--thread-content-margin)\">\n<div class=\"[--thread-content-max-width:40rem] thread-lg:[--thread-content-max-width:48rem] mx-auto max-w-(--thread-content-max-width) flex-1 group\/turn-messages focus-visible:outline-hidden relative flex w-full min-w-0 flex-col agent-turn\" tabindex=\"-1\">\n<div class=\"flex max-w-full flex-col grow\">\n<div class=\"min-h-8 text-message relative flex w-full flex-col items-end gap-2 text-start break-words whitespace-normal [.text-message+&amp;]:mt-1\" dir=\"auto\" data-message-author-role=\"assistant\" data-message-id=\"cd7668c4-c8d2-4c93-997c-1a84784ca1c1\" data-message-model-slug=\"gpt-5-1\">\n<div class=\"flex w-full flex-col gap-1 empty:hidden first:pt-[1px]\">\n<div class=\"markdown prose dark:prose-invert w-full break-words dark markdown-new-styling\">\n<p data-start=\"0\" data-end=\"174\" data-is-last-node=\"\" data-is-only-node=\"\"><strong data-start=\"0\" data-end=\"174\" data-is-last-node=\"\" data-is-only-node=\"\">In this case, immunophenotyping enabled confirmation of a clinically significant lymphoma and, based on literature data, suggested its subtype as T-zone lymphoma (TZL).*<\/strong><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/article>\n<p><i><span style=\"font-weight: 400;\">*Seelig DM, Avery P, et al. Canine T-zone lymphoma: unique immunophenotypic features, outcome, and population characteristics. J Vet Intern Med 2014; 28: 878-86<\/span><\/i><i><span style=\"font-weight: 400;\">.<\/span><\/i><\/p>\n<p>&nbsp;<\/p>\n<p><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/2.1.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2234 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/2.1-300x274.jpg\" alt=\"\" width=\"300\" height=\"274\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/2.2.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2235 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/2.2-300x274.jpg\" alt=\"\" width=\"300\" height=\"274\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/2.3.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2236 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/2.3-300x273.jpg\" alt=\"\" width=\"300\" height=\"273\" \/><\/a>[\/vc_toggle][vc_toggle title=&#8221;Boxer with generalized lymphadenopathy and cytological suspicion of lymphoblastic lymphoma&#8221; css=&#8221;&#8221;]<\/p>\n<ul>\n<li>A 7-year-old Boxer with generalized lymphadenopathy and cytological suspicion of lymphoblastic lymphoma<\/li>\n<li>Cytometric analysis enabled the identification of a clearly dominant population of cells in the examined lymph nodes with the phenotype CD45+ CD3+ CD5+ CD4+, corresponding to helper T lymphocytes.<strong>D\u00edky imunofenotypizaci byla p\u0159\u00edtomnost lymfomu rychle a jednozna\u010dn\u011b potvrzena, nav\u00edc byl jeho p\u016fvod stanoven na T bu\u0148ky.<\/strong> According to the literature, the CD45+ CD4+ phenotype in T-cell lymphomas may predict an aggressive nature and rapid progression of the neoplastic disease, and Boxers are considered a breed with a high predisposition to this type of neoplasia*.**<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><i><span style=\"font-weight: 400;\">*Avery PR, Burton JL, et al. Flow cytometric characterization and clinical outcome of CD4+ T-Cell Lymphoma in dogs: 67 cases. J Vet Intern Med 2014; 28: 538-546.<\/span><\/i><i><span style=\"font-weight: 400;\"><br \/>\n<\/span><\/i><i><span style=\"font-weight: 400;\">**Lurie DM, Milner RJ, et al. Immunophenotypic ad cytomorphologic subclassification of T-cell lymphoma in the boxer breed. Vet Immunology and Immunopathology 2008; 125: 102-110.<\/span><\/i><\/p>\n<p>&nbsp;<\/p>\n<p><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/3.1.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2238 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/3.1-300x274.jpg\" alt=\"\" width=\"300\" height=\"274\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/3.2.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2239 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/3.2-300x273.jpg\" alt=\"\" width=\"300\" height=\"273\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/3.3.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2240 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/3.3-300x273.jpg\" alt=\"\" width=\"300\" height=\"273\" \/><\/a>[\/vc_toggle][vc_toggle title=&#8221;Mixed-breed dog in severe general condition with marked leukocytosis and the presence of atypical cells in a peripheral blood smear&#8221; css=&#8221;&#8221;]<\/p>\n<ul>\n<li>A 9-year-old mixed-breed dog in severe general condition with marked leukocytosis, anemia, thrombocytopenia, and the presence of atypical cells in a peripheral blood smear<\/li>\n<li><span style=\"font-weight: 400;\">As a result of the blood cytometric analysis, a clearly dominant population of stem cells (CD34+) was identified, with concurrent expression of the B-lymphocyte marker (CD79\u03b1+).<br data-start=\"179\" data-end=\"182\" \/>The phenotyped cells did not express CD14 or CD4, which made it highly probable to exclude a myelomonocytic hyperplasia lineage. <strong>In this case, immunophenotyping formed the basis for the final diagnosis of acute B-lymphoblastic leukemia (ALL-B)*.<\/strong><br \/>\n<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><i><span style=\"font-weight: 400;\">* Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction. Vet Immunol Immunopathol 1999; 69: 145-164<\/span><\/i><b><i>.<\/i><\/b><\/p>\n<p>&nbsp;<\/p>\n<p><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/4.1.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2241 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/4.1-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/4.2.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2242 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/4.2-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/4.3.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2243 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/4.3-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/a>[\/vc_toggle][vc_toggle title=&#8221;Miniature Schnauzer showing no clinical symptoms with marked lymphocytosis persisting for several months&#8221; css=&#8221;&#8221;]<\/p>\n<ul>\n<li>A 15-year-old Miniature Schnauzer showing no clinical symptoms, with marked lymphocytosis persisting for several months and no atypical cells present in peripheral blood<\/li>\n<li>In the cytometric blood analysis, the dominant cell population displayed the phenotype CD45+ CD21+ CD79\u03b1+ MHC II+, corresponding to B lymphocytes. At the same time, the lack of expression of the stem cell marker (CD34) in the examined cells excluded the presence of acute lymphoblastic leukemia. <strong>In this case, the immunophenotyping result (in the context of the clinical data and the peripheral blood smear findings) made it possible to consider the presence of chronic B-cell lymphocytic leukemia (B-CLL) or, alternatively, small B-cell lymphoma (B-SLL) with blood involvement*.<\/strong><\/li>\n<\/ul>\n<p><b><\/b><i>*Comazzi S, Gelain ME, Martini V. <\/i><i>Immunophenotype Predicts Survival Time in Dogs with Chronic Lymphocytic Leukemia. JVIM 2011: 25; 100-106.<\/i><\/p>\n<p>&nbsp;<\/p>\n<p><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/5.1.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2244 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/5.1-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/5.2.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2245 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/5.2-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/a><a class=\"dt-pswp-item\" href=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/5.3.jpg\" data-dt-img-description=\"\" data-large_image_width=\"401\" data-large_image_height=\"401\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2246 size-medium\" src=\"https:\/\/vetlab.pl\/wp-content\/uploads\/2021\/08\/5.3-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/a>[\/vc_toggle][vc_toggle title=&#8221;Cat with chronic lymphocytosis of unclear origin&#8221; css=&#8221;&#8221;]<\/p>\n<ul>\n<li>A 12-year-old cat with lymphocytosis persisting for over 2 months and mild atypical features of lymphocytes observed on microscopic examination of the blood smear<\/li>\n<li>Flow cytometric analysis of the patient\u2019s blood sample allowed the identification of a clearly dominant subpopulation of lymphocytes with a CD18+CD5+CD4+ phenotype, corresponding to helper T lymphocytes. According to the literature*, such an immunophenotyping result is strongly suggestive of a neoplastic process of lymphoid lineage (leukemia or lymphoma in a leukemic stage).<\/li>\n<\/ul>\n<p><b><\/b><i>*Rout E.D., et al. Immunophenotypic characterization and clinical outcome in cats with lymphocytosis. Journal of veterinary internal medicine 2020; 34: 105-116.<\/i><\/p>\n<p>&nbsp;<\/p>\n<p><a class=\"dt-pswp-item\" href=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cat-with-chronic-lymphocytosis-of-unclear-origin.jpg\" data-dt-img-description=\"\" data-large_image_width=\"1288\" data-large_image_height=\"453\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-353616\" src=\"https:\/\/veteo.eu\/wp-content\/uploads\/2026\/01\/cat-with-chronic-lymphocytosis-of-unclear-origin.jpg\" alt=\"\" width=\"1288\" height=\"453\" \/><\/a>[\/vc_toggle][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row full_width=&#8221;stretch_row&#8221; gap=&#8221;35&#8243; content_placement=&#8221;middle&#8221; el_id=&#8221;start&#8221; el_class=&#8221;en-st-pt&#8221;][vc_column width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1764926528107{padding-top: 0px !important;}&#8221;][vc_column_text css=&#8221;&#8221;] Immunophenotyping &#8211; a flow cytometry based method now available to Veteo clients The Veteo Laboratory, in cooperation with a team of veterinary oncologists and scientists, offers immunophenotyping of cells using flow cytometry.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1764926534225{padding-top: 0px !important;}&#8221;][vc_single_image image=&#8221;527225&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; equal_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; css=&#8221;.vc_custom_1615022529078{padding-top:&hellip;<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":527411,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-527221","page","type-page","status-publish","hentry","description-off"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.1 - 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